The University of Graz, which was founded in 1585, is Austria’s second oldest university and one of the largest in the country.
* Duration of Contract Temporary employment with option for permanent position
* Temporary Employment 1 year
Your Responsibilities
* Active involvement in research and teaching
* Culture, maintenance, and differentiation of pluripotent stem cells into cardiac cell types (e.g., cardiomyocytes, endothelial cells) as well as generation and culture of cell aggregates in 3D (e.g., organoids, engineered heart tissues)
* Preparation of tissues (cell or nuclear isolation) and cell culture samples for histological, molecular biological, and functional analyses (e.g., protein, RNA, epigenetics, immunostaining, Ca-imaging).
* Isolation and culture of primary cells and standard cell lines; stimulation and genetic modifications (e.g., with CRISPR/Cas9); preparation of tissues (cell or nucleus isolation) and cell culture samples for histological, molecular biological, and functional investigations (e.g., protein, RNA, epigenetics, immunostaining, Ca imaging)
* Sorting and analysis of specific cell populations using flow cytometry (FACS)
* Performing immunostaining, microscopy, and functional studies (e.g., calcium imaging, contraction measurements)
* Preparation of next-generation sequencing libraries (bulk and single-cell; e.g., RNA-seq, ATAC-seq, CUT&Tag, CUT&RUN, HiC, Multiome)
* Conducting molecular biology standard experiments (e.g., Western Blot, qPCR)
* Independent documentation, analysis, and processing of experimental data; development of standard protocols; training of new personnel
* Preparation of reagents, apparatus, and working materials for laboratory exercises (pharmacy studies); co-supervision of students
* General administrative tasks (e.g., ordering and keeping records of chemicals/consumables, equipment maintenance and servicing, obtaining quotes)
Your Profile
* Matura and training as a biological laboratory technician, or a bachelor’s degree in a relevant field (e.g., molecular biology, cell biology, pharmacy), or other equivalent qualifications
* At least 2 years of experience in:
o Cell culture and sterile working methods
o Analytical standard methods (e.g., Western Blot, qPCR, immunostaining, microscopy)
o Planning, execution, evaluation, and presentation of experiments
o Differentiation of pluripotent stem cells into cardiac (e.g., cardiomyocytes, endothelial cells) or other derived cell types; experience in 3D cell culture (e.g., organoids, engineered heart tissue (desired))
o Tissue preparation and flow cytometry (FACS) (desired)
o Preparation of sequencing libraries in bulk and/or single cells (e.g., RNAseq, ATAC-seq, CUT&RUN, CUT&Tag, HiC, scRNA-seq, Multiome, scATAC-seq) (desired)
* Experience in working with students and mentoring new staff (desired)
* Very good English skills
* Analytical thinking, problem-solving skills, thoroughness and precision
* Independent and structured work, team spirit, strong communication skills
* Pedagogical skills and willingness to continue education
* Self-motivation and enjoyment of scientific work
We offer an annual gross salary of at least € 43,838.20 for a full-time position. An overpayment based on qualification and experience is possible.
We Offer
* Meaning: We offer meaningful work for the world of tomorrow.
* A colorful internal continuing education program.
* Collaboration: Interdisciplinary, cross-professional opportunities to work together.
* Benefits: Of course, there are all the usual benefits, from healthcare services to sustainability goals.
* Diversity: A working environment where diversity and inclusion are lived.
* Flexibility: Flexibility in working time models and family-career compatibility.
The University of Graz strives to increase the proportion of women in particular in management and faculty positions and therefore encourages qualified women to apply. In the event of underrepresentation, women with equal qualifications are generally given priority for admission. We welcome applications from persons with disabilities who meet the requirements of the advertised position.
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